Composite

Part:BBa_K3914113

Designed by: Cai PeiJing   Group: iGEM21_SUSTech_Shenzhen   (2021-10-01)


L.reuteri AHL synthesize pathway

This parts is used to produce AHL(acylated homoserine lactones, which is a type of quorum sensing signal molecules) in the L.reuteri as the upstream signal molecule. When cell lysis, these AHL molecules will be released and sent to downstream E.coli. In this parts, promoter ermB is used to activate LuxI gene to promote AHL.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 945
    Illegal EcoRI site found at 975
    Illegal EcoRI site found at 1482
    Illegal EcoRI site found at 1506
    Illegal XbaI site found at 1908
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 945
    Illegal EcoRI site found at 975
    Illegal EcoRI site found at 1482
    Illegal EcoRI site found at 1506
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 945
    Illegal EcoRI site found at 975
    Illegal EcoRI site found at 1482
    Illegal EcoRI site found at 1506
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 945
    Illegal EcoRI site found at 975
    Illegal EcoRI site found at 1482
    Illegal EcoRI site found at 1506
    Illegal XbaI site found at 1908
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 945
    Illegal EcoRI site found at 975
    Illegal EcoRI site found at 1482
    Illegal EcoRI site found at 1506
    Illegal XbaI site found at 1908
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638


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